Device

Part:BBa_K2080014:Design

Designed by: Haojian Li   Group: iGEM16_SCUT-China_B   (2016-10-13)


bcl2-sgRNA-34


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 270
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We design the sequence of the gRNA that is complementary to the bcl-2 promoter region, and try to get closer to the transcription start site to obtain higher repression of bcl-2 trascription.



Source

From BBa_K2080006 

   BBa_K2080007

References